Rag1/Rag2^tm1Mnz
Targeted Allele Detail

Summary

• Symbol: Rag1/Rag2^tm1Mnz
• Name: recombination activating 1; targeted mutation 1, Michel C Nussenzweig
• MGI ID: MGI:3720344
• Synonyms: ASE ko
• Gene: Rag1 Location: Chr2:101468627-101479846 bp, - strand Genetic Position: Chr2, 53.88 cM
• Alliance: Rag1/Rag2^tm1Mnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90487
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Intergenic deletion
• Mutation details: A targeting vector was designed to remove a 7.6 kb genomic segment encoding the antisilencing element (ASE) between the Rag1 and Rag2 genes and replace it with a loxP-flanked neomycin-Cre self excision cassette. Cre was driven by the angiotensin converting enzyme promoter, specific for testis expression. The vector was introduced to E14 129P2/OlaHsd-derived embryonic stem (ES) cells. Excision left a single loxP site in place of the deleted element. Rag1 and Rag2 transcript levels decrease to almost below the detection limit, as measured by RT-PCR. Expression is decreased to a lesser extent in CD4-CD8- thymocytes. Small decreases in mRNA abundance in pro-B and pre-B cells are within the margin of error for the experiments. Rag1 and Rag2 transcript levels decrease to almost below the detection limit, as measured by RT-PCR. Expression is decreased to a lesser extent in CD4-CD8- thymocytes. Small decreases in mRNA abundance in pro-B and pre-B cells are within the margin of error for the experiments. (J:90847)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rag1 Mutation: 123 strains or lines available

References

• Original: J:90487 Yannoutsos N, et al., A cis element in the recombination activating gene locus regulates gene expression by counteracting a distant silencer. Nat Immunol. 2004 Apr;5(4):443-50
• All: 5 reference(s)