Myc^tm39Mnz
Targeted Allele Detail

Summary

• Symbol: Myc^tm39Mnz
• Name: myelocytomatosis oncogene; targeted mutation 39, Michel C Nussenzweig
• MGI ID: MGI:3720515
• Synonyms: Myc^delta
• Gene: Myc Location: Chr15:61857240-61862223 bp, + strand Genetic Position: Chr15, 26.19 cM, cytoband D2-D3
• Alliance: Myc^tm39Mnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145691
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector containing a self-excising loxP-flanked neomycin/sperm-specific Cre cassette was used to delete the promoter, first non-coding exon and a portion of intron 1 of the Mycn gene. This 2.2 Kb deletion removed promoters PO, P1, and P2 while leaving promoter P3 intact. The construct was electroporated into CY2.4 albino C57BL/6J-Tyr^c-2J-derived embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6J-Tyr^c-2J mice to allow for coat-color screening of germline transmission and for self-excision of the neomycin/Cre cassette. RNA-FISH analysis of heterozygote B cells indicated RNA transcription occcured only at one allele compared to the biallelic transcription observed in wild-type B cells. (J:145691)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myc Mutation: 43 strains or lines available

References

• Original: J:145691 Robbiani DF, et al., AID is required for the chromosomal breaks in c-myc that lead to c-myc/IgH translocations. Cell. 2008 Dec 12;135(6):1028-38
• All: 1 reference(s)