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Myctm37Mnz
Targeted Allele Detail
Summary
Symbol: Myctm37Mnz
Name: myelocytomatosis oncogene; targeted mutation 37, Michel C Nussenzweig
MGI ID: MGI:3720516
Synonyms: MycI, MycIsceI
Gene: Myc  Location: Chr15:61857240-61862223 bp, + strand  Genetic Position: Chr15, 26.19 cM, cytoband D2-D3
Alliance: Myctm37Mnz page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:145691
Parent Cell Line:  CY2.4 (ES Cell)
Strain of Origin:  B6(Cg)-Tyrc-2J
Mutation
description
Allele Type:    Targeted (Not Applicable)
Mutation:    Insertion
 
Mutation detailsA targeting vector containing a self-excising lox-P flanked neomycin/sperm-specific Cre cassette was used to introduce a loxP site and an 18 bp I-SceI (S. cerevisiae) endonuclease recognition site (TAGGGATAACAGGGTAAT) into intron 1 of the gene. The construct was electroporated into CY2.4 albino C57BL/6J-Tyrc-2J-derived embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6J-Tyrc-2J mice to allow for coat-color screening of germline transmission and for self-excision of the neomycin/Cre cassette. This mutant locus is left with one loxP site and one I-SceI endonuclease recognition site 5' of exon 2. (J:145691)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myc Mutation:  43 strains or lines available
References
Original:  J:145691 Robbiani DF, et al., AID is required for the chromosomal breaks in c-myc that lead to c-myc/IgH translocations. Cell. 2008 Dec 12;135(6):1028-38
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory