Il4ratm2Tch
Targeted Allele Detail
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Symbol: |
Il4ratm2Tch |
Name: |
interleukin 4 receptor, alpha; targeted mutation 2, Talal A Chatila |
MGI ID: |
MGI:3720593 |
Synonyms: |
IL-4RalphaR576, Il4raR576, Q576R |
Gene: |
Il4ra Location: Chr7:125151443-125178646 bp, + strand Genetic Position: Chr7, 68.94 cM
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Alliance: |
Il4ratm2Tch page
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Lung histology staining of OVA-sensitized control (Q576) and Il4ratm2Tch/Il4ratm2Tch (R576) mice challenged intranasally with OVA on three consecutive days
Show the 4 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:152376
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Parent Cell Line: |
RW-4 (ES Cell)
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Strain of Origin: |
129X1/SvJ
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Allele Type: |
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Targeted |
Mutation: |
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Insertion
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Mutation details: The targeting vector was designed (by site-directed mutagenesis) to change the alanine-guanine to guanine-alanine at basepair 1964-65 of murine IL-4Ra cDNA, corresponding to a single amino acid substitution of glutamine to arginine at codon 576 (Q576R). This also inserted a loxp-flanked PGK-neo cassette within intron 11. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-expressing vector to remove the selection cassette (leaving a single loxp site in intron 11) and then injected into C57BL/6 blastocysts.
(J:152376)
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Generation of the Il4ratm2Tch allele |
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:152376 Tachdjian R, et al., Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma. J Exp Med. 2009 Sep 28;206(10):2191-204 |
All: |
4 reference(s) |
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