Il4ra^tm2Tch
Targeted Allele Detail

Summary

• Symbol: Il4ra^tm2Tch
• Name: interleukin 4 receptor, alpha; targeted mutation 2, Talal A Chatila
• MGI ID: MGI:3720593
• Synonyms: IL-4RalphaR576, Il4ra^R576, Q576R
• Gene: Il4ra Location: Chr7:125151443-125178646 bp, + strand Genetic Position: Chr7, 68.94 cM
• Alliance: Il4ra^tm2Tch page

Lung histology staining of OVA-sensitized control (Q576) and Il4ra^tm2Tch/Il4ra^tm2Tch (R576) mice challenged intranasally with OVA on three consecutive days

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152376
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

Allele Type: Targeted Mutation: Insertion Mutation details: The targeting vector was designed (by site-directed mutagenesis) to change the alanine-guanine to guanine-alanine at basepair 1964-65 of murine IL-4Ra cDNA, corresponding to a single amino acid substitution of glutamine to arginine at codon 576 (Q576R). This also inserted a loxp-flanked PGK-neo cassette within intron 11. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-expressing vector to remove the selection cassette (leaving a single loxp site in intron 11) and then injected into C57BL/6 blastocysts. (J:152376)

Generation of the Il4ra^tm2Tch allele

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il4ra Mutation: 46 strains or lines available

References

• Original: J:152376 Tachdjian R, et al., Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma. J Exp Med. 2009 Sep 28;206(10):2191-204
• All: 4 reference(s)