Cyp1a^tm1Dwn
Targeted Allele Detail

Summary

• Symbol: Cyp1a^tm1Dwn
• Name: cytochrome P450, family 1, subfamily a cluster; targeted mutation 1, Daniel W Nebert
• MGI ID: MGI:3721229
• Synonyms: Cyp1a1/1a2^-, Cyp1a2/Cyp1a1^tm2Dwn, Del(9Cyp1a2-Cyp1a1)1Dwn
• Gene: Cyp1a Location: unknown Genetic Position: Chr9, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122409
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd or 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Cyp1a^tm1Dwn involves 2 genes/genome features (Cyp1a2, Cyp1a1) View all
• Mutation details: The Cyp1a2(t) targeted allele (J:122409) was generated using a targeting vector designed to insert a loxP-flanked PGK-NEO cassette 350 bp downstream of the endogenous stop codon (about 60 bp 3' of exon 7). The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells which were microinjection into C57BL/6 blastocysts. The chimeras were bred with C57BL/6 to generate Cyp1a2(t) mutant mice. To create the Cyp1a1 targeted allele (J:59398), a targeting vector was designed to insert a loxP-flanked hypoxanthine phosphoribosyltransferase (HPRT) minigene in intron 1 and a loxP site downstream of the termination codon in exon 7. Following electroporation into E14TG2a (HPRT-) ES cells and ES cell microinjection into the blastocoele cavity of C57BL/6J embryos, chimeric males were bred with C57BL/6J females. As described in J:86748, these Cyp1a1(t) mutant mice were then bred to a Cre-deleter strain (CAGGS-CRE, mixed C57BL/6J and FVB/NJ genetic background). The resulting transgenic mice found to be heterozygous for the floxed null Cyp1a1(-) allele (containing only exon 1, a portion of intron 1, and one remaining loxP site in the 3' UTR) were bred to mice heterozygous for the Cyp1a2(t) allele (as described in J:122409). Mutant mice (Cyp1a2(t), Cyp1a1(-), CAGGS-CRE) were bred to C57BL/6. Because of the close genomic position of these two genes, offspring having undergone Cre recombinase-mediated interchromosomal recombination between the loxP sites 3' beyond the stop codons of the Cyp1a2 and Cyp1a1 genes could be selected. Such mice were backcrossed to C57BL/6 for 10 generations (while selecting against the Cre-deleter transgene) to generate Cyp1a1/1a2 mutant mice. (J:59398, J:86748, J:122409)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp1a Mutation: 0 strains or lines available

References

• Original: J:122409 Dragin N, et al., Generation of 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-) mouse line. Biochem Biophys Res Commun. 2007 Aug 3;359(3):635-42
• All: 18 reference(s)