Tg(CYP1A1,CYP1A2)1Dwn
Transgene Detail

Summary

• Symbol: Tg(CYP1A1,CYP1A2)1Dwn
• Name: transgene insertion 1, Daniel W Nebert
• MGI ID: MGI:3721236
• Synonyms: hCYP1A1_1A2, BAC-H
• Transgene: Tg(CYP1A1,CYP1A2)1Dwn Location: unknown
• Alliance: Tg(CYP1A1,CYP1A2)1Dwn page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CYP1A1,CYP1A2)1Dwn expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CYP1A1 (1543)

  Cyp1a1

  (MGI:88588)

  human

  CYP1A2 (1544)

  Cyp1a2

  (MGI:88589)

• Mutation details: To create "humanized" CYP1A1 and CYP1A2 transgenic mice (hCYP1A1_1A2), a single copy of the 180-kb human CYP1A1_CYP1A2 locus-containing BAC-H (BAC Human CTB clone 31H21: including the 23.3 kb spacer region, 90 kb of CYP1A2 3'-flanking region and 53 kb of CYP1A1 3' flanking region) was microinjected into (C57BL/6J x DBA/2J)F1 oocytes. Founder mice having a single copy of BAC-H were identified and then backcrossed for 10 generations to C57BL/6 mice. (J:96716)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:96716 Jiang Z, et al., Toward the evaluation of function in genetic variability: characterizing human SNP frequencies and establishing BAC-transgenic mice carrying the human CYP1A1_CYP1A2 locus. Hum Mutat. 2005 Feb;25(2):196-206
• All: 11 reference(s)