Cd28^tm1Shr
Targeted Allele Detail

Summary

• Symbol: Cd28^tm1Shr
• Name: CD28 antigen; targeted mutation 1, Arlene H Sharpe
• MGI ID: MGI:3721239
• Synonyms: Cd28^-
• Gene: Cd28 Location: Chr1:60785547-60812521 bp, + strand Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
• Alliance: Cd28^tm1Shr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68642
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-hygro cassette replaced a 3 kb region encompassing portions of exon 2 and exon 3. The deleted region encoded parts of a IgV-like domain and a transmembrane domain. Fluorescence-activated cell sorting (FACS) analysis showed an absence of protein on naive CD4 T cells isolated from the lymph nodes of homozygous mutant mice. Targeting was performed on an embryonic stem cell clone that was heterozygote for a targeted mutation of Ctla4^tm1Shr. (J:68642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Cd28 Mutation: 52 strains or lines available

References

• Original: J:68642 Mandelbrot DA, et al., B7-dependent T-cell costimulation in mice lacking CD28 and CTLA4. J Clin Invest. 2001 Apr;107(7):881-7
• All: 5 reference(s)