Tg(Gad1-EGFP)G42Zjh
Transgene Detail

Summary

• Symbol: Tg(Gad1-EGFP)G42Zjh
• Name: transgene insertion G42, Z Josh Huang
• MGI ID: MGI:3721280
• Synonyms: GAD67-EGFP, GAD67-GFP (line G42)
• Transgene: Tg(Gad1-EGFP)G42Zjh Location: unknown
• Alliance: Tg(Gad1-EGFP)G42Zjh page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The bacterial artificial chromosome (BAC) clone 56I19 containing the mouse GAD67 gene (and 60 kb of upstream and downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and phosphoglycerate kinase polyadenylation sequence in the first coding exon at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized C57BL/6 mouse eggs. Mice (line G42) selectively express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. Transgene expression (EGFP expression) is detected as early as embryonic day 14 (E14) with high expression levels throughout postnatal development. (J:93895, J:94812)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:93895 Ango F, et al., Ankyrin-based subcellular gradient of neurofascin, an immunoglobulin family protein, directs GABAergic innervation at purkinje axon initial segment. Cell. 2004 Oct 15;119(2):257-72
• All: 106 reference(s)