Gabrg2^tm1Wul
Targeted Allele Detail

Summary

• Symbol: Gabrg2^tm1Wul
• Name: gamma-aminobutyric acid type A receptor, subunit gamma 2; targeted mutation 1, Peter Wulff
• MGI ID: MGI:3721943
• Synonyms: gamma2I77lox
• Gene: Gabrg2 Location: Chr11:41801030-41891684 bp, - strand Genetic Position: Chr11, 24.8 cM
• Alliance: Gabrg2^tm1Wul page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124171
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 4 was replaced with an exon containing a phenylalanine to isoleucine mutation at position 77 (F77I). This point mutation confers drug insensitivity to zolpidem. A loxP site was inserted in the upstream intron and a frt-flanked neomycin cassette followed by another loxP site was inserted in the downstream intron. Chimeric mice were mated with a Flp deleter strain to remove the neomycin cassette. Genomic sequencing of homozygous mice confirmed the presence of the F to I mutation. (J:124171)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabrg2 Mutation: 41 strains or lines available

References

• Original: J:124171 Wulff P, et al., From synapse to behavior: rapid modulation of defined neuronal types with engineered GABAA receptors. Nat Neurosci. 2007 Jul;10(7):923-9
• All: 27 reference(s)