Tg(Pgk1-FGF2)15Cofn
Transgene Detail

Summary

• Symbol: Tg(Pgk1-FGF2)15Cofn
• Name: transgene insertion 15, J Douglas Coffin
• MGI ID: MGI:3722189
• Synonyms: FGF2Tg, TgFGF214-15
• Transgene: Tg(Pgk1-FGF2)15Cofn Location: unknown
• Alliance: Tg(Pgk1-FGF2)15Cofn page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pgk1-FGF2)15Cofn expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FGF2 (2247)

  Fgf2	(MGI:95516)

• Mutation details: Human FGF2 cDNA including the coding sequences for all three CUG and the AUG translational start coding sequences was put under control of a constitutive phosphoglycerate kinase promoter. The Pgk1 polyadenylation sequence was also included in the construct. The construct was microinjected into pronuclear FVB/N oocytes. Production of human FGF2 protein was verified by Western blot. Founder lines with stable copy number and transmission of the transgene were identified and line 15 was established. (J:33064)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:33064 Coffin JD, et al., Abnormal bone growth and selective translational regulation in basic fibroblast growth factor (FGF-2) transgenic mice. Mol Biol Cell. 1995 Dec;6(12):1861-73
• All: 10 reference(s)