Tpsb2^tm1(cre)Adch
Targeted Allele Detail

Summary

• Symbol: Tpsb2^tm1(cre)Adch
• Name: tryptase beta 2; targeted mutation 1, Roberto Adachi
• MGI ID: MGI:3722523
• Synonyms: mMCP-6^-
• Gene: Tpsb2 Location: Chr17:25585282-25587070 bp, + strand Genetic Position: Chr17, 12.53 cM, cytoband A3.3
• Alliance: Tpsb2^tm1(cre)Adch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124554
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129X1/SvJ x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Tpsb2^tm1(cre)Adch expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tpsb2 (MGI:96942)

• Mutation details: The three coding exons downstream of the translation initiation site was replaced with Cre recombinase cDNA. The remaining gene will lack critical amino acids including the codon that encodes the essential catalytic triad residue His75. Absence of wild type protein was confirmed by immunoblot. (J:124554)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tpsb2 (mouse) Summary of all recombinase alleles driven by Tpsb2. Alliance mouse Tpsb2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpsb2 Mutation: 12 strains or lines available

References

• Original: J:124554 Thakurdas SM, et al., The mast cell-restricted tryptase mMCP-6 has a critical immunoprotective role in bacterial infections. J Biol Chem. 2007 Jul 20;282(29):20809-15
• All: 5 reference(s)