Cyp17a1^tm1Vpap
Targeted Allele Detail

Summary

• Symbol: Cyp17a1^tm1Vpap
• Name: cytochrome P450, family 17, subfamily a, polypeptide 1; targeted mutation 1, Vassilios Papadopoulos
• MGI ID: MGI:3722780
• Gene: Cyp17a1 Location: Chr19:46655604-46661439 bp, - strand Genetic Position: Chr19, 38.97 cM
• Alliance: Cyp17a1^tm1Vpap page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:100434
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette replaced the entire gene as well as 3.3 kb sequence upstream and 3.5 kb sequence downstream of the gene. RT-PCR analysis determined that expression of adjacent genes was not affected. Analysis of 129 derived sperm from chimeras showed reduced mRNA and protein expression, as determined by quantitative PCR and western analysis. (J:100434)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp17a1 Mutation: 24 strains or lines available

Notes

Authors state that haploinsufficiency of gene prevents genetic transmission of the mutated allele and generation of non-chimeric heterozygous mice. This phenotype differs from Cyp17a1^tm1Mell heterozygous male mice, which are fertile and have germline transmission of the mutant allele.

References

• Original: J:100434 Liu Y, et al., Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male mice. Mol Endocrinol. 2005 Sep;19(9):2380-9
• All: 2 reference(s)