Tg(Tcra1H3.1,Tcrb1H3.1)1Vire
Transgene Detail

Summary

• Symbol: Tg(Tcra1H3.1,Tcrb1H3.1)1Vire
• Name: transgene insertion 1, Christophe Viret
• MGI ID: MGI:3722813
• Synonyms: 1H3.1 TCR Tg
• Transgene: Tg(Tcra1H3.1,Tcrb1H3.1)1Vire Location: unknown
• Alliance: Tg(Tcra1H3.1,Tcrb1H3.1)1Vire page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcra1H3.1,Tcrb1H3.1)1Vire expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: To generate the I-Ab-restricted CD4+ 1H3.1 T cell hybridoma, a peptide corresponding to residues 52-68 of the a-chain of the I-E MHC class II molecule (Ea52-68) was injected into C57BL/6 mice. T cells expressing the T cell receptor (TCR) combination (Va1-Ja21 and Vb6-Db2.1-Jb2.6) were selected. These TCR sequences were cloned into TCR expression cassettes and the resulting constructs were co-microinjected into (C57BL/6 x SJL)F2 oocytes. Mature T cells from 1H3.1 TCR Tg mice (Va1Vb6) are skewed toward expression of the CD4 coreceptor. A subset of TCRhigh CD8+ T cells (5-10%) is found in the periphery. (J:124574)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:124574 Viret C, et al., Functional and phenotypic evidence for presentation of E alpha 52-68 structurally related self-peptide(s) in I-E alpha-deficient mice. J Immunol. 2000 May 1;164(9):4627-34
• All: 2 reference(s)