Tg(Igk-Eik-H2-Ea^d)1Flv
Transgene Detail

Summary

• Symbol: Tg(Igk-Eik-H2-Ea^d)1Flv
• Name: transgene insertion 1, Richard A Flavell
• MGI ID: MGI:3722860
• Synonyms: Igk-Ea
• Transgene: Tg(Igk-Eik-H2-Ea^d)1Flv Location: unknown
• Alliance: Tg(Igk-Eik-H2-Ea^d)1Flv page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Igk-Eik-H2-Ea^d)1Flv expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	H2-Ea (MGI:95900)

• Mutation details: A 5.2 kb fragment encoding H2-Ea^d was ligated 3' of the Igk enhancer/promoter sequences (Igk-Eik), where the enhancer is a 0.9 kb fragment from the J-C intron. The construct was microinjected into (C57BL/6 x SJL)F2 oocytes. Transgene expression was observed in B-cell areas of the spleen, but density of I-E on spleen and lymph node B-cells was low relative to endogenous I-A or Ig. (J:124907)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:124907 Burkly LC, et al., Clonal deletion of V beta 5+ T cells by transgenic I-E restricted to thymic medullary epithelium. J Immunol. 1993 Oct 15;151(8):3954-60
• All: 2 reference(s)