Sox10^tm4Weg
Targeted Allele Detail

Summary

• Symbol: Sox10^tm4Weg
• Name: SRY (sex determining region Y)-box 10; targeted mutation 4, Michael Wegner
• MGI ID: MGI:3723308
• Synonyms: Sox10^aa1
• Gene: Sox10 Location: Chr15:79039113-79048690 bp, - strand Genetic Position: Chr15, 37.7 cM
• Alliance: Sox10^tm4Weg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124679
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was constructed that contains a Sox10 open reading that changes amino acids 71-73 from CIR to AAA, followed by a loxP flanked neo cassette. The region mutated is necessary for DNA-dependent dimerization of this transcription factor. The vector replaced the entire open reading frame of Sox10 by deleting exons 3 through 5. The neo cassette was removed via in vivo Cre mediated recombination. Quantitative RT-PCR and immunoprecipitation on mid-stage embryos indicated that mRNA and protein levels of the mutated gene were similar to the wild-type gene. (J:124679)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox10 Mutation: 33 strains or lines available

References

• Original: J:124679 Schreiner S, et al., Hypomorphic Sox10 alleles reveal novel protein functions and unravel developmental differences in glial lineages. Development. 2007 Sep;134(18):3271-81
• All: 2 reference(s)