Fosl2^tm2.1Wag
Targeted Allele Detail

Summary

• Symbol: Fosl2^tm2.1Wag
• Name: fos-like antigen 2; targeted mutation 2.1, Erwin F Wagner
• MGI ID: MGI:3757560
• Synonyms: Fosl-2^-, fra-2^delta
• Gene: Fosl2 Location: Chr5:32293145-32315186 bp, + strand Genetic Position: Chr5, 17.33 cM
• Alliance: Fosl2^tm2.1Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125028
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: ES cell containing Fosl2^tm2Wag were transfected with a cre-expressing plasmid to remove the exons 3 and 4 along with a neo cassette. RNase protection and western blot were used to confirm the absence of full-length protein from mouse embryonic fibroblasts using an anti-Fosl2 antibody. However, a fusion protein with the remaining GFP open reading frame is detected using an anti-GFP antibody. (J:125028)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fosl2 Mutation: 13 strains or lines available

References

• Original: J:125028 Eferl R, et al., Simultaneous generation of fra-2 conditional and fra-2 knock-out mice. Genesis. 2007 Jul;45(7):447-51
• All: 8 reference(s)