Tg(Ddx4-cre)1Dcas
Transgene Detail

Summary

• Symbol: Tg(Ddx4-cre)1Dcas
• Name: transgene insertion 1, Diego H Castrillon
• MGI ID: MGI:3757577
• Synonyms: Mvh-Cre, Vasa-Cre
• Transgene: Tg(Ddx4-cre)1Dcas Location: Chr18:85714737-86812993 bp Genetic Position: Chr18, Syntenic
• Alliance: Tg(Ddx4-cre)1Dcas page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Null/knockout, Recombinase)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Tg(Ddx4-cre)1Dcas involves 2 genes/genome features (Neto1, Cbln2) View all
• Tg(Ddx4-cre)1Dcas expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Ddx4

• Mutation details: A 5.6kb fragment of the Ddx4 promoter was ligated to the cre open reading frame with an engineered nuclear localization signal. Eight transgenic lines were created but only two possessed expressing in the gonads as early as E15. When the cre transgene is maternally expressed, all progeny can show ubiquitous recombination even if the cre is not inherited, likely due to perdurance of cre protein in the egg. The transgene integrated into chromosome 18 causing a 1098 Kb deletion and deleting the genes Neto1 and Cbln2. The deletion results in a functional knock-out of both genes in homozygous mice. Founder line 1 has a copy number of greater than 20. (J:125051)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ddx4 (species not specified) Summary of all recombinase alleles driven by Ddx4. Comparative matrix view of recombinase activities

Disease models

Expression

In Mice Carrying this Mutation:

45 assay results 7 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:125051 Gallardo T, et al., Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis. 2007 Jun;45(6):413-7
• All: 180 reference(s)