Arhgef9^tm1.1Betz
Targeted Allele Detail

Summary

• Symbol: Arhgef9^tm1.1Betz
• Name: CDC42 guanine nucleotide exchange factor 9; targeted mutation 1.1, Heinrich Betz
• MGI ID: MGI:3757650
• Synonyms: Collybistin knock-out
• Gene: Arhgef9 Location: ChrX:94092541-94240462 bp, - strand Genetic Position: ChrX, 41.85 cM
• Alliance: Arhgef9^tm1.1Betz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124926
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Transfection of ES cells with a cre construct was used to remove exon 5 along with an inserted neo cassette. Exon 5 encodes part of the catalytic RhoGEF domain. Its deletion and resulting frameshift are predicted to destroy the function of the protein and eliminate the entire C-terminus. The absence of full length and truncated protein products was confirmed by western blot on brain homogenates using either a monoclonal specific for the N-terminus or polyclonal antibody against Arhgef9. (J:124926)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgef9 Mutation: 7 strains or lines available

References

• Original: J:124926 Papadopoulos T, et al., Impaired GABAergic transmission and altered hippocampal synaptic plasticity in collybistin-deficient mice. EMBO J. 2007 Sep 5;26(17):3888-99
• All: 4 reference(s)