Tg(Myh6*/tetO-GCaMP2)1Mik
Transgene Detail

Summary

• Symbol: Tg(Myh6*/tetO-GCaMP2)1Mik
• Name: transgene insertion 1, Michael I Kotlikoff
• MGI ID: MGI:3757978
• Synonyms: ccGCaMP2
• Transgene: Tg(Myh6*/tetO-GCaMP2)1Mik Location: unknown
• Alliance: Tg(Myh6*/tetO-GCaMP2)1Mik page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Myh6*/tetO-GCaMP2)1Mik expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Calm1 (MGI:88251)
  mouse	Mylk (MGI:894806)

• Mutation details: This transgene contains a calcium-sensing molecule, GCaMP2, under the transcriptional control of a minimally active mouse alpha myosin heavy chain promoter fused to seven copies of the tetracycline-responsive promoter element (tetO). GCaMP2 consists of circularly permutated eGFP with the molecule interrupted at residue 145 and a 13-residue peptide of myosin light chain kinase (M13) and calmodulin (CaM), placed at the new N and C termini, respectively. An RSET polyHis peptide was introduced N-terminal to the original methionine in GCaMP1. Mutations resulting in increased brightness (D180Y and V93I), thermal stability (V163A, and S175G) and to prevent GFP dimerization (A206K) were introduced. GCaMP2 is 200 times brighter than GCaMP1. Three GATA sites and two thyroid-like response elements are ablated in Myh6 to make the promoter minimally active. When bred with transgenic mice expressing a tetracycline transactivator or reverse transactivator protein, cardiac-specific expression of GCaMP2 can be controlled by withdrawal or administration of a tetracycline analog. (J:107637)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:107637 Tallini YN, et al., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4753-8
• All: 2 reference(s)