Tamalin^tm2.1Nak
Targeted Allele Detail

Summary

• Symbol: Tamalin^tm2.1Nak
• Name: trafficking regulator and scaffold protein tamalin; targeted mutation 2.1, Shigetada Nakanishi
• MGI ID: MGI:3757981
• Synonyms: Tam^-
• Gene: Tamalin Location: Chr15:101122088-101130637 bp, + strand Genetic Position: Chr15, 56.57 cM, cytoband F3
• Alliance: Tamalin^tm2.1Nak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124994
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Using a targeting vector constructed with C57BL/6 BAC genomic clone, a floxed neo cassette was inserted upstream of exon 5 and an additional lox P site was inserted downstream of exon 7. Cre-mediated recombinated removed the neo cassette and exons 5 through 7 to create a truncated protein detectable by western blot analysis that lacks a complete PDZ dmain and leucine zipper. (J:124994)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tamalin Mutation: 15 strains or lines available

References

• Original: J:124994 Ogawa M, et al., Altered sensitivities to morphine and cocaine in scaffold protein tamalin knockout mice. Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14789-94
• All: 1 reference(s)