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Il7rtm1Abra
Targeted Allele Detail
Summary
Symbol: Il7rtm1Abra
Name: interleukin 7 receptor; targeted mutation 1, Ninan Abraham
MGI ID: MGI:3758859
Synonyms: IL-7Ralpha449F
Gene: Il7r  Location: Chr15:9505874-9530262 bp, - strand  Genetic Position: Chr15, 4.16 cM
Alliance: Il7rtm1Abra page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:125360
Parent Cell Line:  RF8 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted
Mutation:    Other
 
Mutation detailsExons 6 through 8 were replaced with a cDNA encoding exons 6 through 8 with a modification in exon 8 that creates a tyrosine to phenylalanine substitution at amino acid position 449. This substitution disrupts the YXXM motif that when phosphorylated binds to the Src homology 2 domain of PI3 kinase to activate downstream pathways. A floxed neo cassette inserted downstream of exon 8 was removed by cre-mediated recombination. Expression and subcellular localization, as determined by western blot and flow cytometry, are not altered. Downstream signaling through Erk and p38 MAPK are unaffected whereas signaling via STAT5 is affected. (J:125360)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Il7r Mutation:  31 strains or lines available
Notes
Knock in creates a Y449F substitution.
References
Original:  J:125360 Osborne LC, et al., Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice. J Exp Med. 2007 Mar 19;204(3):619-31
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory