Thra^tm1Ffla
Targeted Allele Detail

Summary

• Symbol: Thra^tm1Ffla
• Name: thyroid hormone receptor alpha; targeted mutation 1, Frederic Flamant
• MGI ID: MGI:3758914
• Synonyms: TRalpha1L400R, TRalpha^AMI
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^tm1Ffla page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125353
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, Dominant negative)
• Mutation: Nucleotide substitutions
• Mutation details: The open reading frame was replaced with a construct containing a 5' floxed neo cassette, a cDNA encoding the Thra open reading frame with a nucleotide substitution that results in an amino acid substitution of arginine for leucine at position 400 (L400R), and a 3' IRES-Tau-lacZ cassette. The L400R mutation is thought to place the receptor in an unliganded state and create a dominant negative receptor. Expression of the mutant receptor and lacZ is only achieved by cre-mediated removal of the floxed neo cassette. Western blot analysis was used to confirm expression of the mutant receptor following cre-mediated recombination. (J:125353)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 40 strains or lines available

References

• Original: J:125353 Quignodon L, et al., A Point Mutation in the Activation Function 2 Domain of Thyroid Hormone Receptor {alpha}1 Expressed after CRE-Mediated Recombination Partially Recapitulates Hypothyroidism. Mol Endocrinol. 2007 Oct;21(10):2350-2360
• All: 19 reference(s)