Grb10^Gt(XC302)Byg
Gene trapped Allele Detail

Summary

• Symbol: Grb10^Gt(XC302)Byg
• Name: growth factor receptor bound protein 10; gene trap XC302, BayGenomics
• MGI ID: MGI:3759253
• Synonyms: Grb10^{Gt(beta-geo)2Ward}, Grb10ins7
• Gene: Grb10 Location: Chr11:11880499-11987428 bp, - strand Genetic Position: Chr11, 7.15 cM
• Alliance: Grb10^Gt(XC302)Byg page

Mutation origin

• Mutant Cell Line: XC302
• Germline Transmission: Earliest citation of germline transmission: J:125128
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vector containing the beta-geo cassette was inserted into the intron between exons 6 and 7 creating a truncation. Insertion of the gene trap was confirmed by PCR and Southern blot analysis. Disruption in protein expression was confirmed by western blot. Maternal expression in peripheral tissues such as fat, muscle, liver, pancreas, heart, lung, kidney and spleen is disrupted by insertion of the gene trap. However, paternally inherited expression in the brain is unaffected. (J:125128)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XC302	CC178002 ; (MGI Seq Detail)	5' RACE	Chr11:11901504-11917606 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  24 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	5 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grb10 Mutation: 56 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:125128 Wang L, et al., Peripheral disruption of the Grb10 gene enhances insulin signaling and sensitivity in vivo. Mol Cell Biol. 2007 Sep;27(18):6497-505
• All: 8 reference(s)