Pfkm^{GT(OST56064)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Pfkm^{GT(OST56064)Lex}
• Name: phosphofructokinase, muscle; gene trap OST56064, Lexicon Genetics
• MGI ID: MGI:3759504
• Gene: Pfkm Location: Chr15:97990470-98030328 bp, + strand Genetic Position: Chr15, 54.09 cM
• Alliance: Pfkm^{GT(OST56064)Lex} page

Mutation origin

• Mutant Cell Line: OST56064
• Germline Transmission: Earliest citation of germline transmission: J:125530
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The OmniBank gene trap vector was inserted at position -3,273 to -3,272 relative to the ATG start site. Insertion disrupts the function of the distal promoter as confirmed by RT-PCR. Disruption of protein expression levels was confirmed by western blot analysis wich revealed a 50-75% reduction in the skeletal muscles, little change in the heart and a 99% reduction in the brain. (J:125530)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST56064	CG506496 ; (MGI Seq Detail)	3' RACE	Chr15:98010032-98018808 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfkm Mutation: 31 strains or lines available

References

• Original: J:125530 Richard AM, et al., Tissue-dependent loss of phosphofructokinase-M in mice with interrupted activity of the distal promoter: impairment in insulin secretion. Am J Physiol Endocrinol Metab. 2007 Sep;293(3):E794-801
• All: 3 reference(s)