Mapk1^tm1Kuta
Targeted Allele Detail

Summary

• Symbol: Mapk1^tm1Kuta
• Name: mitogen-activated protein kinase 1; targeted mutation 1, Kunio Takishima
• MGI ID: MGI:3759774
• Synonyms: Erk2(floxN), Erk2^flox(Neo+)
• Gene: Mapk1 Location: Chr16:16801246-16865317 bp, + strand Genetic Position: Chr16, 10.53 cM
• Alliance: Mapk1^tm1Kuta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125594
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A floxed PGK-neo cassette was inserted in reverse direction into intron 1 and an additional loxP site was inserted into intron 3. PCR genotyping was used to confirm insertion. Authors state that this is a hypomorphic allele. Western blot analysis of extracts from brain tissue confirmed a 20% to 40% reduction in protein expression that can be corrected by the removal of the neo cassette. (J:125594)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapk1 Mutation: 43 strains or lines available

References

• Original: J:125594 Satoh Y, et al., Extracellular signal-regulated kinase 2 (ERK2) knockdown mice show deficits in long-term memory; ERK2 has a specific function in learning and memory. J Neurosci. 2007 Oct 3;27(40):10765-76
• All: 3 reference(s)