Gab1^tm4Wbm
Targeted Allele Detail

Summary

• Symbol: Gab1^tm4Wbm
• Name: growth factor receptor bound protein 2-associated protein 1; targeted mutation 4, Walter Birchmeier
• MGI ID: MGI:3759866
• Synonyms: Gab1deltaPI3K (Y448F/Y473F/Y590F)
• Gene: Gab1 Location: Chr8:81491067-81607148 bp, - strand Genetic Position: Chr8, 38.47 cM, cytoband C3
• Alliance: Gab1^tm4Wbm page

Gab1^tm4Wbm/Gab1^tm4Wbm mice have open eyelids at birth and keratinocytes show impaired wound closure in vitro

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125303
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: A cDNA encoding amino acids 198-695 with amino acid substitutions of a tyrosine for a phenylalanine at positions 448, 473 and 590 (Y448F/Y473F/Y590F) and a floxed neo cassette were knocked into exon d. The neo cassette was removed by cre-mediated recombination following a cross to a cre deleter strain (Tg(CMV-cre)1Cgn). The modification to the coding region is thought to interfere with protein-protein interactions with PI3K. Expression of the mutant protein confirmed by western blot analysis on protein extracts from E12 embryos was reduced by 40% to 50% relative to levels in wild-type mice. (J:125303)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gab1 Mutation: 78 strains or lines available

References

• Original: J:125303 Schaeper U, et al., Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo. Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15376-81
• All: 2 reference(s)