Gab1tm5Wbm
Targeted Allele Detail
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Symbol: |
Gab1tm5Wbm |
Name: |
growth factor receptor bound protein 2-associated protein 1; targeted mutation 5, Walter Birchmeier |
MGI ID: |
MGI:3759868 |
Synonyms: |
Gab1deltaShp2 (Y628F) |
Gene: |
Gab1 Location: Chr8:81491067-81607148 bp, - strand Genetic Position: Chr8, 38.47 cM, cytoband C3
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Alliance: |
Gab1tm5Wbm page
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Placenta development and muscle progenitor cell migration are disturbed in Gab1tm5Wbm/Gab1tm5Wbm mice
Show the 1 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:125303
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Parent Cell Line: |
E14.1 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Hypomorph) |
Mutation: |
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Nucleotide substitutions
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Mutation details: A cDNA encoding amino acids 198-695 with an amino acid substitution of tyrosine for phenylalanine at position 628 (Y628F) and a floxed neo cassette were knocked into exon d. The neo cassette was removed by cre-mediated recombination following a cross to a cre deleter strain (Tg(CMV-cre)1Cgn). The modification to the coding region is thought to interfere with protein-protein interactions with Shp2. Expression of the mutant protein confirmed by western blot analysis on protein extracts from E12 embryos was reduced by 40% to 50% relative to levels in wild-type mice.
(J:125303)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gab1 Mutation: |
78 strains or lines available
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Original: |
J:125303 Schaeper U, et al., Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo. Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15376-81 |
All: |
2 reference(s) |
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