F10^tm2Ccmt
Targeted Allele Detail

Summary

• Symbol: F10^tm2Ccmt
• Name: coagulation factor X; targeted mutation 2, Katherine A High
• MGI ID: MGI:3760000
• Synonyms: F10-Friuli
• Gene: F10 Location: Chr8:13087308-13106676 bp, + strand Genetic Position: Chr8, 5.73 cM
• Alliance: F10^tm2Ccmt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145092
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 8 contains the missense mutation encoding Factor X Friuli (Pro343Ser). This allele was generated by targeting the "socket" F10^tm1Ccmt allele with a "plug" that introduced the Friuli mutation and simultaneously eliminated the neomycin resistance gene and reconstituted the Hprt gene, allowing selection of correctly targeted ES cells for HAT resistance and G418 sensitivity. Extrinsic and intrinsic protein activity is decreased to 5% of wild-type. (J:145092)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F10 Mutation: 29 strains or lines available

References

• Original: J:145092 Tai SJ, et al., A viable mouse model of factor X deficiency provides evidence for maternal transfer of factor X. J Thromb Haemost. 2008 Feb;6(2):339-45
• All: 2 reference(s)