Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr
Transgene Detail

Summary

• Symbol: Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr
• Name: transgene insertion AR206, Alexander Y Rudensky
• MGI ID: MGI:3760401
• Synonyms: TCli TCR, Tg(Tcra-V18-Tcra-J, Tcrb-V6-Tcrb-D-Tcrb-J)AR206Ayr
• Transgene: Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr Location: unknown
• Alliance: Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Fragments of Valpha18 (P14A.1)-Jalpha45 and Vbeta6-Dbeta1-Jbeta2.2 were PCR-amplified from genomic DNA of hCLIP:I-Ab-specific T cell hybridoma 51-11.5 grown in H2b (C57BL/6J) mice. The amplified fragments were inserted into separate vectors and co-injected into (C57BL/6 x DBA/2)F2 embryos. Mouse -derived T cell receptor promoters (Tcra, Tcrb) drive expression. This founder line (AR206), carrying co-integrated transgenes, was backcrossed more than 15 times to C57BL/6. (J:125548)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:125548 Wong P, et al., Competition for specific intrathymic ligands limits positive selection in a TCR transgenic model of CD4+ T cell development. J Immunol. 2000 Jun 15;164(12):6252-9
• All: 5 reference(s)