Cd8a^tm1Asin
Targeted Allele Detail

Summary

• Symbol: Cd8a^tm1Asin
• Name: CD8 subunit alpha; targeted mutation 1, Alfred Singer
• MGI ID: MGI:3760403
• Synonyms: CD8.4
• Gene: Cd8a Location: Chr6:71350411-71356155 bp, + strand Genetic Position: Chr6, 32.14 cM, cytoband C
• Alliance: Cd8a^tm1Asin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126026
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was created that includes exons 1-3 that encode the extracellular domain, a floxed neomycin cassette, a chimeric exon 4 encoding the endogenous transmembrane domain ligated to the CD4 cytoplasmic tail, and a truncated version of the noncoding exon 5. The amino acid sequence at the site of gene fusion is IITLICYHR-CRHQQ with the hyphen representing site of gene fusion. Chimeric mice were mated to Cre expressing transgenic mice to induce germline excision of the neomycin-resistance cassette. Expression of the fusion protein was confirmed by immunoprecipitation with anti-CD8 antibodies. (J:126026)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd8a Mutation: 39 strains or lines available

References

• Original: J:126026 Erman B, et al., Coreceptor signal strength regulates positive selection but does not determine CD4/CD8 lineage choice in a physiologic in vivo model. J Immunol. 2006 Nov 15;177(10):6613-25
• All: 9 reference(s)