Tg(UBC-scFv)2Nemz
Transgene Detail

Summary

• Symbol: Tg(UBC-scFv)2Nemz
• Name: transgene insertion 2, David Nemazee
• MGI ID: MGI:3760426
• Synonyms: kappa-macroself, Ulik
• Transgene: Tg(UBC-scFv)2Nemz Location: unknown
• Alliance: Tg(UBC-scFv)2Nemz page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: These mice are designed to express a membrane-tethered single chain variable fragment antibody with specificity to mouse Igk light chain. The 4 kb "Ulik" transgene contained the 1.2 kb human ubiquitin C promoter, rheumatoid factor Ig light chain gene leader exon and first intron, the expressed variable genes from the rat anti-k hybridoma 187 line, the hinge, CH2, and CH3 sequences from rat gamma chain 1 (CH1 domain deleted), and the transmembrane and intracytoplasmic sequences from the H2-kb gene. (J:96759)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:96759 Ait-Azzouzene D, et al., An immunoglobulin C{kappa}-reactive single chain antibody fusion protein induces tolerance through receptor editing in a normal polyclonal immune system. J Exp Med. 2005 Mar 7;201(5):817-28
• All: 7 reference(s)