Tes^{Gt(RRH061)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Tes^{Gt(RRH061)Byg}
• Name: testin LIM domain protein; gene trap RRH061, BayGenomics
• MGI ID: MGI:3760502
• Synonyms: Tes^GT
• Gene: Tes Location: Chr6:17065148-17105824 bp, + strand Genetic Position: Chr6, 7.67 cM
• Alliance: Tes^{Gt(RRH061)Byg} page

Mutation origin

• Mutant Cell Line: RRH061
• Germline Transmission: Earliest citation of germline transmission: J:125765
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector was inserted between exons 3 and 4 disrupting the PET and LIM domains. The absence of a protein product was confirmed by western blot analysis on protein extracts from E10.5 embryos. These allele produces a fusion protein containing 133 amino acids fused to the beta-geo cassette. Since only 32 amino acids of the 104 amino acid PET domain are present in the fusion product this allele is expected to be a null allele. (J:125765)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRH061	CC325750 ; (MGI Seq Detail)	5' RACE	Chr6:17065179-17096377 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

90 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tes Mutation: 32 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:125765 Crompton LA, et al., Early embryonic expression patterns of the mouse Flamingo and Prickle orthologues. Dev Dyn. 2007 Nov;236(11):3137-43
• All: 2 reference(s)