Ptprb^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Ptprb^tm1Dgen
• Name: protein tyrosine phosphatase receptor type B; targeted mutation 1, Deltagen
• MGI ID: MGI:3760508
• Gene: Ptprb Location: Chr10:116111428-116225440 bp, + strand Genetic Position: Chr10, 64.68 cM, cytoband D2
• Alliance: Ptprb^tm1Dgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125783
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ-PGK-neo cassette was inserted between exons 17 and 19 replacing exon 18 and its surrounding intronic sequences. Sequences encoding the first six transmembrain domains are replaced. The predicted protein is fused to a beta-galactosidase peptide and lacks the endogenous transmembrane and cytoplasmic domains. The absence of protein product was confirmed by western blot analysis on protein extracts from embryonic endothelioma cells established from E9.5 mice. (J:125783)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptprb Mutation: 99 strains or lines available

References

• Original: J:125783 Baumer S, et al., Vascular endothelial cell-specific phosphotyrosine phosphatase (VE-PTP) activity is required for blood vessel development. Blood. 2006 Jun 15;107(12):4754-62
• All: 6 reference(s)