Rapgef6^tm1.1Tkat
Targeted Allele Detail

Summary

• Symbol: Rapgef6^tm1.1Tkat
• Name: Rap guanine nucleotide exchange factor (GEF) 6; targeted mutation 1.1, Tohru Kataoka
• MGI ID: MGI:3760543
• Synonyms: RA-GEF-2^-
• Gene: Rapgef6 Location: Chr11:54413673-54590111 bp, + strand Genetic Position: Chr11, 32.13 cM
• Alliance: Rapgef6^tm1.1Tkat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126164
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 21 was removed by cre-mediated deletion following a cross to Tg(ACTB-cre)13Miya mice to create an out of frame deletion. Absence of protein product was confirmed by western blot on protein extracts from lung, thymus, brain, spleen and pancreas. (J:126164)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rapgef6 Mutation: 110 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:126164 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

Phenotypic Similarity to Human Syndrome: Oligoasthenoteratozoospermia (OAT) Syndrome J:218581

References

• Original: J:126164 Yoshikawa Y, et al., The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes. Mol Biol Cell. 2007 Aug;18(8):2949-59
• All: 6 reference(s)