Igh^tm1.1Rsky
Targeted Allele Detail

Summary

• Symbol: Igh^tm1.1Rsky
• Name: immunoglobulin heavy chain complex; targeted mutation 1.1, Klaus Rajewsky
• MGI ID: MGI:3760655
• Synonyms: IgH^mugamma1
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125719
• Parent Cell Line: BALB/c-I (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Deletion, Insertion
• Igh^tm1.1Rsky involves 1 genes/genome features (Igh) View all
• Mutation details: A DNA segment containing a IgH-6 gene (heavy chain of IgM) and an inverted Ighg1 (heavy constant gamma 1 chain) was inserted immediately downstream of the constant heavy chain gene region (CH). To restrict expression of soluble isoforms, the relevant polyA sites were mutated (Ighg1) or eliminated (Igh-6). LoxP sites in opposite orientations were placed around the genes and a neomycin resistance cassette was placed upstream of the entire construct. This construct was used in targeting of stem cells from Igh-6^tm1Fbb mice in which the Igh-6 locus is replaced with a loxP site. Chimeric mice were crossed with transgenic mice expressing Cre recombinase, eliminating the entire CH region and the neo resistance cassette while leaving the mutant IgH-6 gene intact and in the right orientation to express. B cells from these mice were confirmed to exclusively express membrane bound IgM antibody. (J:125719)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:125719 Waisman A, et al., IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig alpha/beta. J Exp Med. 2007 Apr 16;204(4):747-58
• All: 4 reference(s)