Foxn1^tm3(cre)Nrm
Targeted Allele Detail

Summary

• Symbol: Foxn1^tm3(cre)Nrm
• Name: forkhead box N1; targeted mutation 3, Nancy R Manley
• MGI ID: MGI:3760775
• Synonyms: Foxn1^ex9cre
• Gene: Foxn1 Location: Chr11:78248403-78277384 bp, - strand Genetic Position: Chr11, 46.74 cM
• Alliance: Foxn1^tm3(cre)Nrm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125817
• Parent Cell Line: LK-1/LK1 (ES Cell)
• Strain of Origin: C57BL/6NHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Foxn1^tm3(cre)Nrm expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxn1 (MGI:102949)

• Mutation details: An IRES-cre and floxed or frt neo cassette were inserted into the 3' end of exon 9. Insertion was confirmed by PCR of genomic DNA. Removal of the neo cassette by frt-mediated recombination following a cross with Tg(ACTFLPe)9205Dym mice results in a loss of ectopic cre expression but specific expression is retained. RT-PCR was used to confirm that endogenous gene expression was unaffected. (J:125817)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxn1 (mouse) Summary of all recombinase alleles driven by Foxn1. Comparative matrix view of recombinase activities Alliance mouse Foxn1 gene page

Expression

In Mice Carrying this Mutation:	4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available
• Carrying any Foxn1 Mutation: 106 strains or lines available

References

• Original: J:125817 Gordon J, et al., Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. BMC Dev Biol. 2007;7:69
• All: 78 reference(s)