Runx1^tm1Yg
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1Yg
• Name: runt related transcription factor 1; targeted mutation 1, Yoram Groner
• MGI ID: MGI:3760788
• Synonyms: P2^neo, P2neo, Runx1^P2neo
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1Yg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125830
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin resistance cassette was inserted, in opposite transcriptional orientation, at nucleotide 27675861 of the mouse Chr 16 genomic sequence (NCBI Ref Seq NT-039625), ~1 kb upstream of the transcription initiation site from the P2 (more proximal) transcriptional promoter in exon 2. In E16.5 kidney and P1.5 tongue, where transcription normally occurs from both the P1 (more distal) and P2 promoters, only P1-derived transcripts are detected by RT-PCR in homozygous mutant tissues; E16.5 stomach epithelium, which normally employs only the P2 promoter, expresses no Runx1 transcripts in homozygous mutants. In thymus, RT-PCR and immunohistochemistry confirm the lack of mRNA and protein expression in mutant embryos at E15.5 and its gradual recovery by E16.5-E17.5 as the transition from the P2 to the P1 promoter occurs. (J:125830)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

Notes

Removal of the neo cassette by cre-mediated recombination following crosses with either Tg(Pgk1-cre)1Lni or Tg(EIIa-cre)C5379Lmgd mice resulted in a reversion back to a phenotype indistunguishable from wild-type phenotype (Runx1^P2loxP or P2^loxP).

References

• Original: J:125830 Pozner A, et al., Developmentally regulated promoter-switch transcriptionally controls Runx1 function during embryonic hematopoiesis. BMC Dev Biol. 2007;7:84
• All: 3 reference(s)