Unc5b^tm1Matl
Targeted Allele Detail

Summary

• Symbol: Unc5b^tm1Matl
• Name: unc-5 netrin receptor B; targeted mutation 1, Marc Tessier-Lavigne
• MGI ID: MGI:3760978
• Synonyms: Unc5b^bGal
• Gene: Unc5b Location: Chr10:60598373-60667360 bp, - strand Genetic Position: Chr10, 31.52 cM, cytoband B4
• Alliance: Unc5b^tm1Matl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126240
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A secretory trap vector possessing a splice acceptor followed by a linker protein, a transmembrane domain, beta-geo, an IRES domain, and a placental alkaline phosphatase reporter gene was targeted to intron 2 of the gene locus. As a result of integration, exons 1 and 2 splice to the vector sequences resulting in a mutant fusion protein with no biological activity. Northern blots of RNA from E10.5 embryos indicated no wild-type transcript while RT-PCR indicated very low levels (under 3%) of wild-type transcript. (J:126240)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Unc5b Mutation: 63 strains or lines available

References

• Original: J:126240 Lu X, et al., The netrin receptor UNC5B mediates guidance events controlling morphogenesis of the vascular system. Nature. 2004 Nov 11;432(7014):179-86
• All: 8 reference(s)