Vegfatm3Gne
Targeted Allele Detail
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| Symbol: |
Vegfatm3Gne |
| Name: |
vascular endothelial growth factor A; targeted mutation 3, Genentech |
| MGI ID: |
MGI:3761357 |
| Gene: |
Vegfa Location: Chr17:46327919-46343295 bp, - strand Genetic Position: Chr17, 22.79 cM
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| Alliance: |
Vegfatm3Gne page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:126080
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Humanized sequence) |
| Mutation: |
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Nucleotide substitutions
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Mutation details: Ten amino acid substitutions were made in exons 3, 4 and 5 to convert the sequence to a human sequence such that arginine is replaced with a histidine at position 26 (R26H), alanine with glycine at 57 (A57G), arginine with a glycine at 64 (A64G), serine with glutamic acid at 71 (S71E), serine with glycine at 87 (S87G), serine with asparagine at 99 (S99N), arginine with lysine at 100 (R100K), threonine with alanine at 110 (T110A), lysine with arginine at 111 (K111R) and proline to glutamine at 112 (P112Q). A floxed neo cassette used as a selectable marker was removed by expression of cre recombinase. ELISA assays were used to confirm the binding of A4.6.1 to hum-X VEGFA protein expressed from ES cells.
(J:126080)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Vegfa Mutation: |
38 strains or lines available
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| Original: |
J:126080 Gerber HP, et al., Mice expressing a humanized form of VEGF-A may provide insights into the safety and efficacy of anti-VEGF antibodies. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3478-83 |
| All: |
2 reference(s) |
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Analysis Tools
