Diaph1^tm1.1Sna
Targeted Allele Detail

Summary

• Symbol: Diaph1^tm1.1Sna
• Name: diaphanous related formin 1; targeted mutation 1.1, Shuh Narumiya
• MGI ID: MGI:3761497
• Synonyms: mDia1^-
• Gene: Diaph1 Location: Chr18:37976654-38068529 bp, - strand Genetic Position: Chr18, 19.71 cM
• Alliance: Diaph1^tm1.1Sna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126090
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1 and a neo cassette flanked by frt sites and containing a 3' loxP site was inserted downstream of exon 1. The neo cassette and exon 1 was removed by cre-mediated recombination following a cross to Tg(EIIa-cre)C5379Lmgd mice. Insertion and removal of the neo cassette and exon 1 was confirmed by PCR analysis on genomic DNA. Absence of protein was confirmed by western blot analysis on brain, lung and thymus homogenate. (J:126090)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Diaph1 Mutation: 116 strains or lines available

References

• Original: J:126090 Sakata D, et al., Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1. J Exp Med. 2007 Sep 3;204(9):2031-8
• All: 8 reference(s)