Cyld^tm1.1Awai
Targeted Allele Detail

Summary

• Symbol: Cyld^tm1.1Awai
• Name: CYLD lysine 63 deubiquitinase; targeted mutation 1.1, Ari Waisman
• MGI ID: MGI:3761624
• Synonyms: CYLD^ex7/8
• Gene: Cyld Location: Chr8:89423675-89478573 bp, + strand Genetic Position: Chr8, 43.51 cM
• Alliance: Cyld^tm1.1Awai page

Enlarged spleen and Peyer's patches in Cyld^tm1.1Awai/Cyld^tm1.1Awai mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126113
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 7 was removed by cre-mediated recombination and results in an out of frame translation. The absence of a wild-type allele in mouse embryonic fibroblasts was confirmed by RT-PCR using primers located in exon 6 and 9. However, higher levels of an alternative splice form without exons 7 and 8, which is missing the TRAF2 and NEMO binding sites, are produced. (J:126113)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyld Mutation: 44 strains or lines available

References

• Original: J:126113 Hovelmeyer N, et al., Regulation of B cell homeostasis and activation by the tumor suppressor gene CYLD. J Exp Med. 2007 Oct 29;204(11):2615-27
• All: 6 reference(s)