Serpinb1a^tm1.1Cben
Targeted Allele Detail

Summary

• Symbol: Serpinb1a^tm1.1Cben
• Name: serine (or cysteine) peptidase inhibitor, clade B, member 1a; targeted mutation 1, Charaf Benarafa
• MGI ID: MGI:3761627
• Synonyms: serpinb1
• Gene: Serpinb1a Location: Chr13:33026075-33035168 bp, - strand Genetic Position: Chr13, 13.75 cM, cytoband A4
• Alliance: Serpinb1a^tm1.1Cben page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125918
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was introduced into intron 6 and a floxed Hyg-TK selection cassette into intron 7. Exon 7 and the selection cassette were removed by transient Cre expression, leaving a single loxP site behind. Western blot analysis on bone marrow neutrophils from homozygote mice indicated there was no expression of the wild-type allele. (J:125918)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Serpinb1a Mutation: 28 strains or lines available

References

• Original: J:125918 Benarafa C, et al., The neutrophil serine protease inhibitor serpinb1 preserves lung defense functions in Pseudomonas aeruginosa infection. J Exp Med. 2007 Aug 6;204(8):1901-9
• All: 15 reference(s)