Pcna^tm1Jcbs
Targeted Allele Detail

Summary

• Symbol: Pcna^tm1Jcbs
• Name: proliferating cell nuclear antigen; targeted mutation 1, Heinz Jacobs
• MGI ID: MGI:3761720
• Synonyms: PCNA^K164, PCNA^K164R
• Gene: Pcna Location: Chr2:132091206-132095100 bp, - strand Genetic Position: Chr2, 64.15 cM
• Alliance: Pcna^tm1Jcbs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126595
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: An A to G transition was targeted at exon 4 of the locus to mutate residue 164 from a lysine to an arginine (K164R). For targeting, a Frt-flanked PGK selection cassette was inserted into intron 1 with one loxP site upstream of the selection cassette. Due to the presence of several psuedogenes that make targeting difficult, homologous recombination of ES cells was verified by multiplex ligation-dependent probe amplification using primers with intronic sequence. (J:126595)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcna Mutation: 16 strains or lines available

References

• Original: J:126595 Langerak P, et al., Rapid and quantitative detection of homologous and non-homologous recombination events using three oligonucleotide MLPA. Nucleic Acids Res. 2005;33(22):e188
• All: 5 reference(s)