Tg(H2-K-TcrbP14)128Mak
Transgene Detail

Summary

• Symbol: Tg(H2-K-TcrbP14)128Mak
• Name: transgene insertion 128, Tak W Mak
• MGI ID: MGI:3762014
• Synonyms: VB8.1, Vbeta8.1
• Transgene: Tg(H2-K-TcrbP14)128Mak Location: unknown
• Alliance: Tg(H2-K-TcrbP14)128Mak page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-K-TcrbP14)128Mak expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcrb (MGI:98578)

• Mutation details: A recombined Tcrb gene was cloned from the CD8-positive T cell clone P14, which is specific for lymphocytic choriomeningitis virus. The gene contained Vbeta8.1, Dbeta, Jbeta2.4 and Cbeta2 gene segments and the corresponding cDNA was put downstream of the H-2K promoter. Downstream of the Tcrb coding sequence, a genomic fragment of the human beta globulin gene and an IgH enhancer were added to introduce splicing sites, polyA sequence and to enhance expression. Of the four founding strains (125, 126, 128, 132) line 128 demonstrated the highest expression by flow cytometry analysis and was chosen for further study. (J:111265)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:111265 Pircher H, et al., T cell tolerance to Mlsa encoded antigens in T cell receptor V beta 8.1 chain transgenic mice. EMBO J. 1989 Mar;8(3):719-27
• All: 16 reference(s)