Psmd4^tm2Smta
Targeted Allele Detail

Summary

• Symbol: Psmd4^tm2Smta
• Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; targeted mutation 2, Shigeo Murata
• MGI ID: MGI:3762169
• Synonyms: Rpn10a
• Gene: Psmd4 Location: Chr3:94939999-94949880 bp, - strand Genetic Position: Chr3, 40.74 cM
• Alliance: Psmd4^tm2Smta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126546
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A version of the open reading frame with exons 7 through 10 replaced by a floxed cDNA fragment encoding amino acids 219 to 376 and neo cassette followed a modified exon 7 with a stop codon and polyadenylation signal attached at the 3' end was knocked into the gene. These modifications would direct the expression of only the 'a' alternative transcript instead of the full range transcipts, 'a' through 'e'. Modifications to the genomic DNA were confirmed by Southern blot analysis and PCR genotyping. The presence of only one splice variant was confirmed by RT-PCR and western blot analysis on proteins extracted from the liver. (J:126546)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Psmd4 Mutation: 25 strains or lines available

References

• Original: J:126546 Hamazaki J, et al., Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development. Mol Cell Biol. 2007 Oct;27(19):6629-38
• All: 1 reference(s)