Psmd4^tm2.1Smta
Targeted Allele Detail

Summary

• Symbol: Psmd4^tm2.1Smta
• Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; targeted mutation 2.1, Shigeo Murata
• MGI ID: MGI:3762171
• Synonyms: Rpn10deltaUIM
• Gene: Psmd4 Location: Chr3:94939999-94949880 bp, - strand Genetic Position: Chr3, 40.74 cM
• Alliance: Psmd4^tm2.1Smta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126546
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated recombination was used to remove the floxed fragment containing a cDNA that encodes amino acids 219 to 376 and a neo cassette leaving behind a modified exon 7 with a stop codon and polyadenylation site. This removed the UIM domains from the protein. PCR genotyping was used to confirm the removal of the cDNA fragment and neo cassette. (J:126546)

Expression

In Mice Carrying this Mutation:

65 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psmd4 Mutation: 25 strains or lines available

References

• Original: J:126546 Hamazaki J, et al., Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development. Mol Cell Biol. 2007 Oct;27(19):6629-38
• All: 1 reference(s)