Ccl19^tm1Cys
Targeted Allele Detail

Summary

• Symbol: Ccl19^tm1Cys
• Name: C-C motif chemokine ligand 19; targeted mutation 1, Jason G Cyster
• MGI ID: MGI:3762257
• Synonyms: Ccl19^-, Ccl19^tm1Salu
• Gene: Ccl19 Location: Chr4:42754525-42756558 bp, - strand Genetic Position: Chr4, 22.44 cM
• Alliance: Ccl19^tm1Cys page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126345
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Nucleotides 38 through 120 of the second exon were replaced with a stop codon followed by an IRES-EGFP and a floxed neo cassette. The neo cassette was subsequently removed by cre-mediated recombination following a cross to mice carrying the cre gene driven by a beta-actin promoter (allele not specified). The absence of protein product was confirmed by western blot analysis on proteins extracted from the lymph nodes and spleen. (J:126345)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ccl19 Mutation: 7 strains or lines available

Notes

The gene targeting removed one CCL19 genes (the one with an atg start codon; CCL19a) but not the transcribed pseudogenes of CCL19 (acg start codon; CCL19b, CCL19c). Most or all CCL19 protein is gone in the mutant mice.

References

• Original: J:126345 Link A, et al., Fibroblastic reticular cells in lymph nodes regulate the homeostasis of naive T cells. Nat Immunol. 2007 Nov;8(11):1255-65
• All: 8 reference(s)