Ity2a^MOLF/EiJ
QTL Variant Detail

Summary

• QTL variant: Ity2a^MOLF/EiJ
• Name: immunity to S. typhimurium 2a; MOLF/EiJ
• MGI ID: MGI:3762332
• Synonyms: Ity2a^MOLF/Ei
• QTL: Ity2a Location: Chr11:37768580-60402716 bp Genetic Position: Chr11, cM position of peak correlated region/allele: 23.57 cM
• QTL Note: genome coordinates based on the boundaries of the QTL region

Variant origin

• Strain of Specimen: MOLF/EiJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers resistance to infection by Salmonella typhimurium compared to C57BL/6J. (J:126552)
• Inheritance: Not Specified

Notes

Ity2a and Ity2b appear to both be necessary for resistance to S. typhimurium.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:126552

Previously identified Salmonella susceptibility QTLs Ity2 and Ity3 were confirmed and refined by linkage analysis of 232 (C57BL/6J x MOLF/EiJ)F2 animals. Parental strain MOLF/EiJ is susceptible to Salmonella typhimurium infection compared to parental strain C57BL/6J. Ity2 and Ity3 were further analyzed using subcongenic lines and candidate genes were identified using gene expression profiling after infection with S. typhimurium.

Ity2 was refined to an interval on mouse Chromosome 11 between D11Mit112 (32 cM) and Mpo (49 cM). Peak linkage occurred at Nos2 (45.6 cM) with LOD=7.8. Ity/Ity2 congenic animals were constructed by selecting for MOLF/EiJ-derived donor DNA at D1Mcg4 (Ity) and D11Mit84 - Mpo (Ity2) on a C57BL/6J genetic background. This line was then bred into two different subcongenic lines, RecD and RecI. At least two different loci were found to contribute to S. typhimurium resistance and were named Ity2a and Ity2b. Ity2a maps to a 23 Mb interval from D11Mit109 (19 cM) to D11Mit26 (33.3 cM) while Ity2b maps to a 13 Mb interval from D11Mit5 (37 cM) to D11Mit8 (46 cM). Gene expression analysis identified Havcr2 as a promising candidate gene for Ity2a. No candidate genes were identified for Ity2b.

Ity3 was refined to an interval on mouseChromosome 1 between D1Mit135 (59.7 cM) and D1Mit201 (79 cM) with peak linkage of LOD=4.1 occurring between D1Mit218 (67 cM) and D1Mit100 (71.5 cM). Ity/Ity3 congenic animals were constructed by selecting for MOLF/EiJ-derived donor DNA at D1Mcg4 (Ity) and D1Mit135 - D1Mit17 (Ity3) on a C57BL/6J genetic background. This line displayed significantly reduced survival time after S. typhimurium infection. Subcongenic lines RecA, RecB, RecC, and RecE were constructed and localized the Ity3 critical region to a34 Mb interval between D1Mit193 (68 cM) and D1Mit63 (87.8 cM). Gene expression analysis identified Lax1 and Chi3l1 as potential candidate genes for Ity3.

References

• Original: J:126552 Sancho-Shimizu V, et al., Molecular genetic analysis of two Loci (ity2 and ity3) involved in the host response to infection with salmonella typhimurium using congenic mice and expression profiling. Genetics. 2007 Oct;177(2):1125-39
• All: 1 reference(s)