Cacnb2^tm1Mfre
Targeted Allele Detail

Summary

• Symbol: Cacnb2^tm1Mfre
• Name: calcium channel, voltage-dependent, beta 2 subunit; targeted mutation 1, Marc Freichel
• MGI ID: MGI:3762346
• Synonyms: Cavbeta2^flox
• Gene: Cacnb2 Location: Chr2:14607899-14992719 bp, + strand Genetic Position: Chr2, 10.7 cM
• Alliance: Cacnb2^tm1Mfre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126506
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 16 was flanked with loxP sites and a PGK-neo cassette with a loxP site was inserted downstream of exon 6. The neo cassette was removed by cre-mediated recombination following a cross to Tg(EIIa-cre)C5379Lmgd mice. Insertions into the genomic DNA were confirmed by Southern blot analysis. (J:126506)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacnb2 Mutation: 50 strains or lines available

References

• Original: J:126506 Weissgerber P, et al., Reduced cardiac L-type Ca2+ current in Ca(V)beta2-/- embryos impairs cardiac development and contraction with secondary defects in vascular maturation. Circ Res. 2006 Sep 29;99(7):749-57
• All: 3 reference(s)