Rr320^tm1.1Fier
Targeted Allele Detail

Summary

• Symbol: Rr320^tm1.1Fier
• Name: regulatory region 320; targeted mutation 1.1, Steven Fiering
• MGI ID: MGI:3762391
• Synonyms: delta5'HS2c, Hbb-ar^tm1.1Fier
• Gene: Rr320 Location: Chr7:103501973-103526752 bp, + strand Genetic Position: Chr7, Syntenic
• Alliance: Rr320^tm1.1Fier page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126615
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout, RMCE-ready)
• Mutation: Intragenic deletion
• Mutation details: A 1194 bp fragment spanning -10,415 to -9221 relative to the start site of mouse Hbb-y was placed into a CMV-hyTK cassette with the selectable marker flanked by inverted loxP sites and inserted into the genome by homologous recombination. Recombinase-mediated cassette exchange (RMCE) was used to create an allele without the core sequence but with the flanking sequences intact by transient expression of cre. Southern blot analysis was used to confirm the modification. (J:126615)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr320 Mutation: 0 strains or lines available

Notes

ES cell line = DS2B

References

• Original: J:126615 Hu X, et al., Deletion of the core region of 5' HS2 of the mouse beta-globin locus control region reveals a distinct effect in comparison with human beta-globin transgenes. Blood. 2006 Jan 15;107(2):821-6
• All: 1 reference(s)